PCR

Polymerase chain reaction- a highly sensitive form of serology testing which detects DNA particles of an organism.

Repeated Chlamydia pneumonia infection, persistence, caridovascular disease, luteolin

Submitted by Jim K on Wed, 2006-05-31 07:45

I don't believe we have this linked to our Research Pages (Marie?). This is a brilliant dissertation from the Finnish group, some of the world's experts on Cpn as some of the faculty in Helsinki were part of the original group who discovered the very existence of Cpn.

This dissertation demonstrates a number of important findings:

  • Repeated infection with Cpn "...induced persistent chlamydial DNA and inflammation in lung tissue and development of mouse Hsp60 autoantibodies."
  • Repeated infection with Cpn "...significantly increased subendothelial lipid accumulation in the aortic sinus area."
  • That "A flavonoid, luteolin, was shown to effectively decrease the chlamydial load and inflammatory reactions in lung tissue." Note: luteolin is not the same as lutein.
  • Conventional antimicrobial treatments are not effectively to eradicate persistent infection.
Go to the link and you can download the whole thing in pdf form.

Experimental Chlamydia pneumoniae infection model: effects of repeated inoculations and treatment

Liisa Törmäkangas

Lääketieteellinen tiedekunta, Oulun yliopisto

PCR Analysis

Submitted by mrhodes40 on Wed, 2005-09-07 16:56

Here is a link to a detailed and technical paper on PCR analysis that evaluates and outlines the problem with culturing CPn and the variations in abilities in different labs. It also talks about the results of split samples and concordance between some PCR approaches and discordance with others. Though this is highly technical, it answers the question of how can it be that there are still some researchers producing papers saying, for example, that they tested patients with MS for CPn and found the incidence to be no higher than controls, such as this article here but note that this one used antibody tests, a ridiculously ineffective way of looking for CPn in cryptic form in the brain.

Results, and Clinical Performance of Five PCR Assays for Detecting Chlamydia pneumoniae DNA in Peripheral Blood Mononuclear Cell

Submitted by Jim K on Sun, 2005-09-04 10:45

J. B. Mahony,1,2,* S. Chong,1 B. K. Coombes,1 M. Smieja,1 and A. Petrich1,2
Hamilton Regional Laboratory Medicine Program, St. Joseph's Hospital,1 and Department of Pathology and Molecular Medicine, McMaster University,2 Hamilton, Ontario, Canada
Link here

Received 18 January 2000/Returned for modification 31 March 2000/Accepted 4 May 2000

Chlamydia pneumoniae has been associated with atherosclerosis and coronary artery disease (CAD), and its DNA has been detected in atheromatous lesions of the aorta, carotid, and coronary arteries by a variety of PCR assays. The objective of this study was to compare the performances of five published PCR assays in the detection of C. pneumoniae in peripheral blood mononuclear cells (PBMCs) from patients with coronary artery disease.