27 Apr 2018
Author
dee_hates_cpn
Title

Concerning microscopy, at what magnification can we view EB particles & inclusion bodies?

Body

How many doctors/patients utilize this valuable tool? Do any physicians or patients examine their own blood, other bodily fluids, or a scraping of epithelial cells from the inner cheek?  Has anyone actually identified any of these CPN structures?  Without dye?  At what magnification? Have any progressive changes been noted, as to a decreasing number of inclusion bodies over time, with CAP? Isn't this a potentially useful means of ascertaining how the CAP is progressing? Related question: How long after killing intracellular (active) CPN can we expect cell apoptosis?

Comments

Dee, since chlamydophila pneumonia is a largely intracellular pathogen, you would be very lucky to find a few elementary bodies floating around in your sputum or blood: that is why an accurate reading is so hard.Also, if you look at this page: http://www.cpnhelp.org/introduction_the_the_hand, elementary bodies are very, very small................... SarahA Journey through Light and Shadow

Completed Stratton/Wheldon regime for aggressive secondary progressive MS in June 2007, after four years, three of which intermittent.   Still improving bit by bit and no relapses since finishing treatment.

I know this post is old.. butI think I found them with this microscopehttp://store.amscope.com/t490b-dk.htmlI put some pictures in this thread and some video.  I purchased a 89.99 adapter so I can use my SLR to take pics and video.  I also bought the amscope on amazon.com .. Cant believe you can buy stuff like that there!http://www.cpnhelp.org/can_anyone_tell_me_if_chl I use the 40x optical with 20x maginifcation to see those pictures.  They are crystal clear through the lens, it is hard to focus through two devices to actually record.  There is a 100x oil immersion lens that lets you get closer, but the actual antigen is so small and Im just an amatuer! I can get much much closer on an invaded RBC though.  I havent used a microscope since biology lab.. so I cant get closer images dialed down yet.  Once you put the drop of oil on to look, you ruin the slide so I stopped trying and am happy with the magnification I can see.  What I believe is the actualy bacteria of cpn, looks like a little fleck, almost like a fly buzzing around the cell.  I have seen them actually invaginate a RBChttp://i47.tinypic.com/34pn7rt.jpghttp://i46.tinypic.com/2pu0je9.jpg