MediTest
Submitted by mrhodes40 on Mon, 2007-05-28 18:56

J Neurosci Res. 2003 Mar 1;71(5):740-50. Links
Chlamydia pneumoniae infection promotes the transmigration of monocytes through human brain endothelial cells.MacIntyre A, Abramov R, Hammond CJ, Hudson AP, Arking EJ, Little CS, Appelt DM, Balin BJ.
Department of Biomedical Sciences, Philadelphia College of Osteopathic Medicine, Philadelphia, Pennsylvania, USA.

We have investigated the effects of Chlamydia pneumoniae on human brain endothelial cells (HBMECs) and human monocytes as a mechanism for breaching the blood-brain barrier (BBB) in Alzheimer's disease (AD). HBMECs and peripheral blood monocytes may be key components in controlling the entry of C. pneumoniae into the human brain. Our results indicate that C. pneumoniae infects blood vessels and monocytes in AD brain tissues compared with normal brain tissue. C. pneumoniae infection stimulates transendothelial entry of monocytes through HBMECs. This entry is facilitated by the up-regulation of VCAM-1 and ICAM-1 on HBMECs and a corresponding increase of LFA-1, VLA-4, and MAC-1 on monocytes. C. pneumoniae infection in HBMECs and THP-1 monocytes up-regulates monocyte transmigration threefold in an in vitro brain endothelial monolayer. In this way, C. pneumoniae infection in these cell types may contribute to increased monocyte migration and promote inflammation within the CNS resulting from infection at the level of the vasculature. Thus, infection at the level of the vasculature may be a key initiating factor in the pathogenesis of neurodegenerative diseases such as sporadic AD. Copyright 2002 Wiley-Liss, Inc.

PMID: 12584732 [PubMed - indexed for MEDLINE

Comments

This is not too new but we did not have it in research pages so I grabbed it. Though this was undertaken to support the AD/CPn model, it is pertinent to MS because the work shows that CPn impacts the BBB and allows immune cells in. Since we know CPn infected immune cells are common it seems obvious CPn can get into the brain. There goes the old "the BBB is impervious" argument! If you go to the research pages, click the Multiple Sclerosis page and read the annotations, you can also look at the page we have on there that shows microglia can be infected with CPn. marie On CAP since Sept '05 for MS, RA, Asthma, sciatica. EDSS at start 5.5. Currently on: Doxy 200, Azith 3x week, Tini cont. since April '07, all supplements. "Color out side the lines!"

On CAP since Sept '05 for MS, RA, Asthma, sciatica. EDSS at start 5.5.(early cane) Now 6 (cane full time) Originally on: Doxy 200, Azith 3x week, Tini cont. over summer '07, Revamp of protocol in Summer '08 by Stratton due to functional loss; clarithro

Question, probably simple. Is it not the lack of Vitamin D that allows the blood-brain barrier to go down and allow the bacteria in?

The difference between what we do and what we are capable of doing would suffice to solve most of the world’s problems.  Mohandas Gandhi

The difference between what we do and what we are capable of doing would suffice to solve most of the world’s problems. Mohandas Gandhi

Mac               I'm not an expert, not on the level of DW, Norman, Eric, Red, etc.; however, based on what I know from years of reading about this stuff and what I've read here, the reason bacteria get's in is likely multi-factoral.  In addition to vitamin D, there's also the issue of fatty acids (fa).  Depending on diet, the quantity of healthy fatty acids used by your body to manufacture things such as myelin for instance, may not be the most useful and resilient kind towards repelling bacteria.  There are likely other things that I'm not thinking of at the moment but may come to me after I've thought about it, but I do have the opinion that it's not a cut and dry reason but probably a combination of several things that just happen at about the same time.all my bestJohnRRMS/EDSS was 4.5, now 4.??? on Wheldon Protocol (nac, doxycycline, azithromycin, metronidazole) since 04/12/2006

best, JohnRRMS/EDSS was 4.5, 5, 6, 6.5, 6.9999, 6.5 on Wheldon/Stratton Protocol beginning 04/12/2006nac 4x600 mg/daydoxycycline 2x100mg/dayazithromycin 3x250mg/day MWFmetronidazole 3x400mg/day then 3x500mg/day