Biofilm Testing

I picked up a comment in another post (by K23la) regarding Biofilm Testing and it perked my interest.  I 'm not sure what advantage it would be to know specifically what pathogens are hiding in the biofilms we have  as the removal process is, I believe, the same. Perhaps some of the more scientifically-minded  here may have input.   

The test  is available at Fry Labs in Arizona and your own prescribing doctor can order it (the test kit will then be sent free).  If you order it yourself, the test kit is $15.00.

The cost of  the Fluorescent DNA Stain is $350.00.   All information is available at

"This cutting edge test is not available anywhere else and uses highly specific DNA dyes to aid in visualizing infections and blood-borne biofilm communities. A photograph and a report will be generated of the laboratory’s findings"




   I read the same post as you from K23la about Fry Labs, thank you K23la, and went to the web site.  It turns out it is in my city and I called the office curious about testing for biofilm.  In conversation with their office they said they have good results with the detection of biofilm from their tests. They recommended the Advanced Stain Tests panel numbers 87206 and 87205, both for $350.00.  They prefer that antibiotic use be stopped for 2 weeks prior to the blood draw so I have to think about that.  As you stated the test kit was sent to my immunologist for free as I will do the blood draw there and hand carry it to Fry Labs for testing if I decide to take it.  In recent discussion with my immunologists as well as the person at Fry Labs the issue of biofilm is "heating up" in the world of infection and my immunologists thought it made a lot of sense in his experience. 

Cpn, Mycoplasma, Chronic EBV, M.S.(MRI, Spinal Tap-greater than 5 oligoclonal bands and VEP), PANDAS(OCD). Wheldon CAP (started 12/08), Azithromycin/Clarithromycin(12/09), Lithium, Lamictal, NAC(2.4g/day), D3(15,000IU/day)

Hi, Moxy.. thanks for your input!    I was surprised no one else had commented on this considering the fact many here are l now "discovering" our biofilm issues as a possible hinderance to our treatment.

I called them as well before posting but didn't elaborate my conversation, so thank you for providing those points about stopping the ABX.  Does your immunologist think it's worthwhile to have done? 




JeanneRoz ~ DX'd w/ CPN 4/2007; 6/07 -"officially" dx'd w/CFIDS/FM; also: HHV6, EBV, IBS-C, 100 Doxy:BID; 500 mg Biaxin BID; Tindamax Pulses, B12 shots, ERFA Dessicated Thyroid,Cortef, Iodoral 25 mg, Vit D-6,000 uni

Thanks for posting this information on biofilms. I am at such an early stage of WP that I am still watching and learning and in my own challenge just trying to titrate up to the required level of medications so that I get to the pulses- I watch with interest and read along the way, of these sorts of issues that i may need to address at a later stage. Thanks for keeping me in the loop of information flow. Suzanne
FMS/ME dx 2001. Started Wheldon Protocol 16 Jan. '10. Mino 100mg q 24 h. Roxy 150mg q 12h. Cholestyramine, LDN 0.75mg q 24 h. prophylactic migraine-topamax 75mg q 24h. migraines, headache, fatigue, sleep problems, body aches

This information (about biofilms) is something I wish I had known of at the beginning of my treatment.  I believe many had heard of, and were aware, of "biofilms", (it's especially prevalent in discussions on the Lyme and Autism boards) but we (or at least "I") didn't correlate it to our CPN treatment.

I have also been told by some here that these (addressing the gut/yeast/metal and biofilm issues first to the clear the way to treat the CPN)  are now being addressed first in CPN patients before starting them on ABX by certain doctors.

So much information has transpired here in the 3 years I've been on board.... we just keep learning..........




JeanneRoz ~ DX'd w/ CPN 4/2007; 6/07 -"officially" dx'd w/CFIDS/FM; also: HHV6, EBV, IBS-C, 100 Doxy:BID; 500 mg Biaxin BID; Tindamax Pulses, B12 shots, ERFA Dessicated Thyroid,Cortef, Iodoral 25 mg, Vit D-6,000 uni

Jeanne, I started treatment once before and didn't realize how important it was to maintain a healthy gut. To me, it was a matter of taking my probiotics and antibiotics and so on. The biofilms has started me on a diffrent track of treatment and it's been more difficullt. Many are fortunate not to have yeast or gut issues and don't have to do biofim treatments.

Thanks for posting this information on testing. I am not sure if I can have it done at this point, due to expences. It would be interesting to know what I have.



started Wheldon cap 4/21/08 for Cpn, CMV, EBV, CFS. Cap hold 4/09vascultis. Restart 9/09 with Dr.Powell, restore gut, 4000 D3, supplements,  Pos. Bartonella and Babesia, Rifampin 600 mgs and Biaxon 1 gram. 

I had a biofilm test done at Fry Labs and have heard that often there is cpn in the biofilm found ( generally, not for me).

So I could find your page here and I think I belong here as well.

The biofilm discussion is still new and Dr. F might come up with new things it is not a test for people who want only very scientific old tests.

It helped me a lot. But he also finds bugs which are not treatable at the moment. I think it is interesting what he finds.

Especially if people have MS or ALS like symptoms.

There is good discussion on lymenet concerning the biofilm but only very few memmbers speak about the newest

information. He will also advice your Dr. with options for treatment. It helped me a lot.

In the biofilm are also many viruses and that might be a reason we are not feeling well.


K23la, cpn is intracellular pathogen, so it can not be found in extracellular biofilms except for its non metabolizing EB state, which does not cause direct damage to the host. The same with many viruses. I read few information about intracellular biofilms, but I suppose they talk only about extracellular. Also what are non treatable bugs for example and how did they found out?

Stratton/Wheldon protocol 02/2006 - 10/11 for CFS and many problems 30 years

Pathogenic bacteria known to reside in biofilms include:

Borrelia burgdorferi, Escherichia coli, Candida albicans, Clostridium difficile, Clostridium perfringens, Helicobacter pylori, Klebsiella pneumoniae, Legionella pneumophila, Listeria monocytogenes, Pseudomonas aeruginosa, Salmonella typhimurium, Staphylococcus aureus, Staphylococcus epidermidis, and Vibrio cholerae.

The number of human diseases shown to be associated with biofilms is expanding and includes chronic bacterial prostatitis, chronic rhinosinusitis, cystic fibrosis pneumonia, infective endocarditis, periodontitis, recurrent otitis media, and virtually all device and implant related infections. Strong evidence is also beginning to emerge for an etiologic role of pathogenic mucosal biofilms in gastrointestinal diseases, such as Irritable Bowel Disorders: Crohn’s disease and ulcerative colitis.

JeanneRoz ~ DX'd w/ CPN 4/2007; 6/07 -"officially" dx'd w/CFIDS/FM; also: HHV6, EBV, IBS-C, 100 Doxy:BID; 500 mg Biaxin BID; Tindamax Pulses, B12 shots, ERFA Dessicated Thyroid,Cortef, Iodoral 25 mg, Vit D-6,000 uni